Chemical analysis

There is a great deal of interest in characterizing small particles such as bacteria, extracellular vesicles, and viruses by flow cytometry. The common availability, as well as the multiparameter and high throughput capabilities of flow cytometry, make this technology attractive for measuring small particles. However, many of the flow cytometers currently being used are not able to detect biological particles smaller than 600nm, in fact very few can detect biological particles 300nm or less.

Monoclonal antibodies (mAbs) remain one of the fast-growing classes of biopharmaceuticals and are significantly improving the quality of life for patients all around the world. For greater understanding of bioprocess product quality attributes, subunit analysis is quick, simple, and automatable. Here’s a full workflow for automated sample preparation and LCMS analysis of mAbs obtained directly from complex bioreactor samples.