G-protein-coupled receptors (GPCRs) represent a ubiquitous membrane protein family and are important drug targets. Their diverse signaling pathways are driven by complex pharmacology arising from a conformational ensemble rarely captured by structural methods.
Understanding the signaling pathways that biomarkers can activate can aid in the development of checkpoint immunotherapies. Surface Plasmon Resonance (SPR) is a common technique used to identify and elucidate biomarker interactions, signaling pathways, mechanisms of interaction, drug discovery, and the development of therapeutics. It provides a label-free, real-time method for determining binding kinetics and binding affinity of key biomolecular interactions. Systems like Alto from Nicoya Lifesciences, the world’s first digital SPR instrument to integrate digital microfluidics and nanotechnology, can measure biomolecules in pM concentrations in various sample matrices with high specificity while offering the versatility of working with a range of biomolecule types.
Pressure-Induced Protein Aggregation Detection: Protein aggregation is a recognized signal of instability and can lead to the loss of protein function. It is therefore crucial to detect protein aggregation early in the drug development process to inform further drug development decisions. Pressure, a stressor used for generating aggregates by impacting noncovalent interactions without the need to change temperature or solvents1, was employed to create aggregated protein for this spiked study.
Biosimilar molecules are designed to mimic innovator molecules that have been approved and are available on the market, providing others within the industry an opportunity to create structurally similar therapeutic medicines for greater patient accessibility. This application note explores the success of Microfluidic Modulation Spectroscopy (MMS) as an automated tool for detecting and differentiating small changes in secondary structure and predicting overall stability of biosimilars for their long-term stability studies.
Liquid biopsy is a useful tool during longitudinal monitoring of NSCLC patients but requires highly sensitive and reliable technologies for accurate detection of genomic alterations. Circulating tumor DNA (ctDNA) analysis has clinical utility in EGFR mutant NSCLC, while Circulating tumor cells (CTCs) consist a unique source of information at the cellular level. Digital PCR (dPCR) is a valuable tool for accurate and valid analysis of gene mutations in liquid biopsy analysis. The scope of the present study was to evaluate the presence and estimate mutation allele frequencies (MAFs) of EGFR mutations in plasma cfDNA and corresponding CTC-derived gDNA using Crystal Digital PCRâ„¢, in a group of EGFR mutant NSCLC patients under osimertinib therapy at two time points (before treatment initiation and at progression of disease).
The naica® system, a flexible digital PCR solution for assay development: application to wastewater-based epidemiology of COVID-19.
Stilla Technologies has introduced into the market the naica® system, using Crystal Digital PCR™ technology for the precise and highly sensitive quantification of nucleic acids. This powerful, yet easy-to-use digital PCR (dPCR) platform is the highest-plexing dPCR instrument available, with six fluorescent channels used to detect multiple genetic targets in a single run.
Scientists devoted to immuno-oncology are establishing new ways to target and destroy cancer cells through personalized immunotherapies. Thanks to decades of research and advances in technologies, scientists have richly documented the human adaptive and innate immune response mechanisms and are now using that information to design and administer personalized immunotherapies.
Today’s lab is a high data throughput environment. Nearly all of your equipment is capturing data, and key analytical systems are capturing more data than ever before. As the pace, volume and variety of data increase, the use of analytics has become essential to creating operational efficiencies
With SARS-CoV2, many people were left wondering about the need for multiple shots, whereas certain vaccines, such as the vaccine for yellow fever, require only one shot in our lifetime. In short, it’s due to a combination of escape variants and dropping levels of antibodies allows for reinfection.
Delivering Growth – in Asia and Beyond.
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